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Using Micropipettes

Examine your micropipette. At the top it has a plunger that you can push in to empty the micropipette; next to the plunger is an ejector you can use to eject the plastic tip from the end of the micropipette. Along the side, it has a volume adjustment wheel you can use to adjust the volume the pipette will take up or contain.

Look at the volume dial along the side of the micropipette. Micropipettes measure volumes in microliters. Determine what the volume is set to at present and adjust that volume with the volume adjustment wheel to reach the appropriate or desired volume.

Insert the end of the micropipette shaft into one of the plastic tips in your plastic tip box. Do not handle the plastic tip with your fingers.

Depress the plunger with your thumb until you reach the first stop.

Insert the plastic tip of the pipette just below the surface of the fluid or water in your beaker.

Release your thumb pressure on the plunger, slowly and gently, drawing fluid into the plastic tip of the micropipette. Once the plunger has traveled all the way out, remove the pipette tip from the solution.

Transfer the pipette to the receiving vessel/beaker/microfuge tube and place the tip just below the surface of the fluid in the receiving vessel. Do not submerge it completely.

Depress the plunger slowly and gently to expel all the fluid in the micropipette tip. This time, continue to apply pressure past the first stop until you reach the second stop.

Remove the pipette tip from the solution. Then release your thumb pressure on the plunger of the pipette.

Follow your lab’s protocol for disposing of the micropipette tips.

Warning

Never, ever, draw fluid up into a Pasteur or volumetric pipette by sucking on it with your mouth. Some chemists and biologists did this “back in the day,” and predictably enough it sometimes caused serious accidents.

 

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PIPETTE TYPES

Pipettes came in two varieties: volumetric and measuring. Volumetric pipettes are designed to transfer a specific , predetermind volume of liquis. they resemble simple glass tibes and cannot be used to accurately measure liquid amounts less than their specified capacity. Measuring pipettes , on the other hand are calibrated with small divisions and are often adjustable , allowing user to accurately draw up however much liquid they desire. Measuring pipettes when transferring incredibly small volumes of liquid.

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CLEANING PIPETTES

Pipettes require cleaning after every use to ensure that they stay accurate and to prevent contamination from any previous contents. To clesn one draw distilled water into the pipette and till ti , so that the water makes contact with the insude surface of the pipette. Repeat this process twice , then rinse the entire pipette with distilled water to finish cleaning it.

Using Pipettes

Regardless of the type of pipette being used, using them takes care and attention. To prevent damage when drawing in a liquid, place the pipette 1/4th of an inch from the bottom of your container. Then place your finger over the end or gently squeeze the bulb at the end, depending on the type of pipette. When the required volume has been drawn up, gently tap the side of the pipette to remove excess droplets. Then, hold the pipette at a 10 to 20 degree angle when dispensing. Do not blow through a pipette to remove excess liquid.

Pipettes

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Quality Assurance : 

                             Since the establishment of our company, we have aimed at offering our clients with the best quality products. For this, we use talented technical team for best quality products. Further, being an ISO 9001:2008 certified company; quality is of great importance to us. Our company Singh Science Systems employs a team of quality controllers who check our products on the following parameters:

    • Accuracy
    • Readability
    • Repeatability
    • Performance
    • Dimensional accuracy
    • Weight
    • Packing etc

Mini Size Fix Volume Micro Pipette in Giza

Whatsapp No. +919266169686

MODEL CAPACITY IN
Mini-5 5µl
Mini-10 10µl
Mini-20 20µl
Mini-25 25µl
Mini-50 50µl
Mini-100 100µl
Mini-200 200µl
Mini-250 250µl
Mini-500 500µl
Mini-1000 1ml

Salient Features of fully Autoclavable Fix Volume Mini Micropipettes

Fully autoclave, accurate & precise.

Mini size pipette optimum 130mm. gives maximum accuracy.

Two step plungers ensure last drop dispending.

Poly propylene material.

Silicon rubber oring.

Steel tampered spring.

Smart tip corn design allows perfect fit for 200µl as well as 20µl tips.

& allows perfect fit for 1000µl as well as 250µl tips also.

Two year warranty for manufacturing defects (T. C. Apply)

Mini Size Fix Volume Micro Pipette in Alexandria

Whatsapp No. +919266169686

MINI SIZE FIX VOLUME MICRO PIPETTE

MODEL CAPACITY IN
Mini-5 5µl
Mini-10 10µl
Mini-20 20µl
Mini-25 25µl
Mini-50 50µl
Mini-100 100µl
Mini-200 200µl
Mini-250 250µl
Mini-500 500µl
Mini-1000 1ml

Salient Features of fully Autoclavable Fix Volume Mini Micropipettes

Fully autoclave, accurate & precise.

Mini size pipette optimum 130mm. gives maximum accuracy.

Two step plungers ensure last drop dispending.

Poly propylene material.

Silicon rubber oring.

Steel tampered spring.

Smart tip corn design allows perfect fit for 200µl as well as 20µl tips.

& allows perfect fit for 1000µl as well as 250µl tips also.

Two year warranty for manufacturing defects (T. C. Apply)